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FACS analysis of mouse hematopoietic stem cell subsets – gating strategy | Stem Cell Assays
I’d like to share my experience with isolation and analysis of mouse hematopoietic stem cells by surface phenotype using flow cytometry.
Historically, a lot of methods was proposed for isolation and identification of hematopoietic stem cells (HSC). We can identify HSC based on surface or functional markers or using combined approaches.
The case I’m presenting below:
mouse strain: C57B6/J (WT)
age: 12 weeks
tissue: bone marrow
